Publications

2017
Kaye B, Yoo TY, Foster PJ, Yu C-H, Needleman DJ. Bridging length scales to measure polymer assembly. Mol. Biol. Cell. 2017;28 :1379-1388. Download Paper
Kaye B, Foster PJ, Yoo TY, Needleman DJ. Developing and Testing a Bayesian Analysis of Fluorescence Lifetime Measurements. PLoS ONE. 2017;12 (1) :e0169337. doi:10.1371/journal.pone.0169337. Download Paper
2016
Wu H-Y, Nazockdast E, Shelley MJ, Needleman DJ. Forces positioning the mitotic spindle: Theories, and now experiments. BioEssays [Internet]. 2016 :1600212–n/a. Publisher's Version Download Paper
Oh D, Yu C-H, Needleman D. Spatial organization of the Ran pathway by microtubules in mitosis. Proceedings of the National Academy of Sciences. 2016. Download
Farhadifar R, Ponciano JM, Andersen EC, Needleman DJ, Baer CF. Mutation Is a Sufficient and Robust Predictor of Genetic Variation for Mitotic Spindle Traits in Caenorhabditis elegans. Genetics [Internet]. 2016. Publisher's VersionAbstract

Different types of phenotypic traits consistently exhibit different levels of genetic variation in natural populations. There are two potential explanations: either mutation produces genetic variation at different rates, or natural selection removes or promotes genetic variation at different rates. Whether mutation or selection is of greater general importance is a longstanding unresolved question in evolutionary genetics. We report mutational variances (VM) for 19 traits related to the first mitotic cell division in C. elegans, and compare them to the standing genetic variances (VG) for the same suite of traits in a worldwide collection C. elegans. Two robust conclusions emerge. First, the mutational process is highly repeatable: the correlation between VM in two independent sets of mutation accumulation lines is  0.9. Second, VM for a trait is a good predictor of VG for that trait: the correlation between VM and VG is  0.9. This result is predicted for a population at mutation-selection balance; it is not predicted if balancing selection plays a primary role in maintaining genetic variation.

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Yoo TY, Needleman DJ. Studying Kinetochores In Vivo Using FLIM-FRET. The Mitotic Spindle: Methods and Protocols. 2016 :169–186. Download Paper
Hu H, Juvekar A, Lyssiotis CA, Lien EC, Albeck JG, Oh D, Varma G, Hung YP, Ullas S, Lauring J, et al. Phosphoinositide 3-Kinase Regulates Glycolysis through Mobilization of Aldolase from the Actin Cytoskeleton. Cell. 2016;164 :433-446. Download Paper
2015
Foster PJ, Fürthauer S, Shelley MJ, Needleman DJ. Active contraction of microtubule networks. eLife. 2015 :10.7554/eLife.10837. Download Paper
Needleman D. The Material Basis of Life. Trends in cell biology. 2015. Download Paper
Farhadifar R, Baer CF, Valfort A-C, Andersen EC, Müller-Reichert T, Delattre M, Needleman DJ. Scaling, selection, and evolutionary dynamics of the mitotic spindle. Current Biology. 2015;25 :732–740. Download Paper
2014
Sazer S, Lynch M, Needleman D. Deciphering the Evolutionary History of Open and Closed Mitosis. Current Biology. 2014;24 :R1099–R1103. Download Paper
Needleman D, Brugues J. Determining physical principles of subcellular organization. Developmental cell. 2014;29 :135–138. Download Paper
Brugués J, Needleman D. Physical basis of spindle self-organization. Proceedings of the National Academy of Sciences. 2014;111 :18496–18500. Download Paper
Farhadifar R, Needleman D. Automated segmentation of the first mitotic spindle in differential interference contrast microcopy images of C. elegans embryos. In: Mitosis. Springer ; 2014. pp. 41–45. Download Paper
Yu C-H, Langowitz N, Wu H-Y, Brugues J, Needleman D. Measuring Microtubule Polarity in Spindles with Second-Harmonic-Generation Microscopy. Biophysical Journal. 2014;106 :1578-1587. Download Paper
Ojeda-Lopez MA, Needleman DJ, Song C, Ginsburg A, Kohl PA, Li Y, Miller HP, Wilson L, Raviv U, Choi MC, et al. Transformation of taxol-stabilized microtubules into inverted tubulin tubules triggered by a tubulin conformation switch. Nature materials. 2014;13 :195–203. Download Paper
2013
Needleman D. Developing cell biology. eLife. 2013;2 :e00571. Download Paper
Needleman DJ, Ojeda-Lopez MA, Raviv U, Miller HP, Li Y, Song C, Feinstein SC, Wilson L, Choi MC, Safinya CR. Ion specific effects in bundling and depolymerization of taxol-stabilized microtubules. Faraday discussions. 2013;166 :31–45. Download Paper
2012
Brugués J, Nuzzo V, Mazur E, Needleman DJ. Nucleation and transport organize microtubules in metaphase spindles. Cell. 2012;149 :554–564. Download Paper

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