Farhadifar R, Ponciano JM, Andersen EC, Needleman DJ, Baer CF. Mutation Is a Sufficient and Robust Predictor of Genetic Variation for Mitotic Spindle Traits in Caenorhabditis elegans. Genetics [Internet]. 2016;203 (4) :1859-1870. Publisher's VersionAbstract

Different types of phenotypic traits consistently exhibit different levels of genetic variation in natural populations. There are two potential explanations: either mutation produces genetic variation at different rates, or natural selection removes or promotes genetic variation at different rates. Whether mutation or selection is of greater general importance is a longstanding unresolved question in evolutionary genetics. We report mutational variances (VM) for 19 traits related to the first mitotic cell division in C. elegans, and compare them to the standing genetic variances (VG) for the same suite of traits in a worldwide collection C. elegans. Two robust conclusions emerge. First, the mutational process is highly repeatable: the correlation between VM in two independent sets of mutation accumulation lines is  0.9. Second, VM for a trait is a good predictor of VG for that trait: the correlation between VM and VG is  0.9. This result is predicted for a population at mutation-selection balance; it is not predicted if balancing selection plays a primary role in maintaining genetic variation.

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Yoo TY, Needleman DJ. Studying Kinetochores In Vivo Using FLIM-FRET. The Mitotic Spindle: Methods and Protocols. 2016;(1413) :169–186. Download Paper
Hu H, Juvekar A, Lyssiotis CA, Lien EC, Albeck JG, Oh D, Varma G, Hung YP, Ullas S, Lauring J, et al. Phosphoinositide 3-Kinase Regulates Glycolysis through Mobilization of Aldolase from the Actin Cytoskeleton. Cell. 2016;164 :433-446. Download Paper
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Needleman D. The Material Basis of Life. Trends in cell biology. 2015. Download Paper
Farhadifar R, Baer CF, Valfort A-C, Andersen EC, Müller-Reichert T, Delattre M, Needleman DJ. Scaling, selection, and evolutionary dynamics of the mitotic spindle. Current Biology. 2015;25 :732–740. Download Paper
Sazer S, Lynch M, Needleman D. Deciphering the Evolutionary History of Open and Closed Mitosis. Current Biology. 2014;24 :R1099–R1103. Download Paper
Needleman D, Brugues J. Determining physical principles of subcellular organization. Developmental cell. 2014;29 :135–138. Download Paper
Brugués J, Needleman D. Physical basis of spindle self-organization. Proceedings of the National Academy of Sciences. 2014;111 :18496–18500. Download Paper
Farhadifar R, Needleman D. Automated segmentation of the first mitotic spindle in differential interference contrast microcopy images of C. elegans embryos. In: Mitosis. Springer ; 2014. pp. 41–45. Download Paper
Yu C-H, Langowitz N, Wu H-Y, Brugues J, Needleman D. Measuring Microtubule Polarity in Spindles with Second-Harmonic-Generation Microscopy. Biophysical Journal. 2014;106 :1578-1587. Download Paper
Ojeda-Lopez MA, Needleman DJ, Song C, Ginsburg A, Kohl PA, Li Y, Miller HP, Wilson L, Raviv U, Choi MC, et al. Transformation of taxol-stabilized microtubules into inverted tubulin tubules triggered by a tubulin conformation switch. Nature materials. 2014;13 :195–203. Download Paper
Needleman D. Developing cell biology. eLife. 2013;2 :e00571. Download Paper
Needleman DJ, Ojeda-Lopez MA, Raviv U, Miller HP, Li Y, Song C, Feinstein SC, Wilson L, Choi MC, Safinya CR. Ion specific effects in bundling and depolymerization of taxol-stabilized microtubules. Faraday discussions. 2013;166 :31–45. Download Paper
Brugués J, Nuzzo V, Mazur E, Needleman DJ. Nucleation and transport organize microtubules in metaphase spindles. Cell. 2012;149 :554–564. Download Paper
Oh D, Zidovska A, Xu Y, Needleman DJ. Development of time-integrated multipoint moment analysis for spatially resolved fluctuation spectroscopy with high time resolution. Biophysical journal. 2011;101 :1546–1554. Download Paper
Safinya CR, Raviv U, Needleman DJ, Zidovska A, Choi MC, Ojeda-Lopez MA, Ewert KK, Li Y, Miller HP, Quispe J, et al. Nanoscale assembly in biological systems: from neuronal cytoskeletal proteins to curvature stabilizing lipids. Advanced Materials. 2011;23 :2260–2270. Download Paper
Needleman DJ, Farhadifar R. Mitosis: Taking the Measure of Spindle Length. Current Biology. 2010;20 :R359–R360. Download Paper
Brugués J, Needleman DJ. Nonequilibrium fluctuations in metaphase spindles: Polarized light microscopy, image registration, and correlation functions, in OPTO. International Society for Optics and Photonics ; 2010 :76180L–76180L. Download Paper
Mirny LA, Needleman DJ. Quantitative characterization of filament dynamics by single-molecule lifetime measurements. Methods in cell biology. 2010;95 :582–600. Download Paper